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Georgios A. Spyroulias, PhD.Department of Pharmacy
University of Patras
Panepistimioupoli-Rion,
GR-26504 Patras, GREECE
Tel: +30.2610.969950 (office)
+30.2610.969951 (terra silico)
+30.2610.969952 (terra vitro)
Fax: +30.2610.969950
Email: G.A.Spyroulias@upatras.gr
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Profile
 Polypeptides tend to become organized independently into a variety of functional scaffolds and to socialize with other partner molecules, forming small biomolecular complexes or large assemblies, in various intersections of biological pathways. Proteins’ architecture, plasticity and interaction determine the fate of a variety of biological events. Understanding the functional role of peptides and proteins is strongly coupled to the atomic-level insights of their structure and flexibility.The research activities of the group encompass the study of the structure and dynamics of peptides and proteins involved in signalling pathways, proteolysis and ion/metal transfer, both in silico and in vitro. |
Terra Vitro
Terra Vitro is the wet biology lab for protein production, labelling, purification and characterization. NMR study of proteins and protein complexes requires the production and isolation of the molecules labelled in 13C and 15N active NMR nuclei. Proteins with a molecular weight ranging from 8 to 22 kDa (70 to 200 amino acids) are currently over-expressed in high yields in our lab using bacterial and eukaryotic systems and purified/isolated accordingly.
The lab offers a variety of expression vectors and is equipped with all the appropriate facilities for expression, purification and analysis of recombinant native or mutated proteins, bearing a variety of purification tags.
Terra Silico
Terra Silico is the computer lab for NMR data analysis and Biomolecular Simulations. NMR data provide an atomic-level information for the structure, dynamics, and interaction of polypeptides and can drive Structure-Activity Relationship studies through the design of protein/peptide analogues or protein substrates. Biomolecular Simulations are applied to study the conformational dynamics of protein-protein /peptide /substrate complexes.
Terra silico is equipped with powerful computational systems running software for NMR data analysis, structure calculations, docking and molecular dynamics simulation, structure validation etc.
GR-NMR
An NMR-related site presenting the Greek groups and their activities in the field. There is a number of teams in various universities and institutes that are applying NMR spectroscopy for conformational studies and for analytical purposes. NMR instruments are settled down in Universities of Athens, Thessaloniki, Ioannina, Crete and Patras, as well as research institutes at Athens (NCSR Demokritos, National Hellenic Research Foundation; NHRF) with the higher field magnet being 600 MHz (U of Athens and NHRF). The research activities of the Greek NMR groups are oriented to the analysis of natural products and drug candidates, the conformational properties of peptides and proteins and food quality analysis. EAST-NMR
EAST-NMR is an FP7 EU-funded project. It has the specific aim to enhance the access to NMR facilities and the NMR services to East European users towards an efficient and coordinated pan-European pool of NMR capacities to enable collaborative research and technological achievements. EAST-NMR will combine activities, such as: (i) Networking, (ii) Trans-national access, and (iii) Joint research activities, in a co-ordinated manner. University of Patras is the Greek partner (Partner #12) to EAST-NMR project and is participating to a variety of activities such as, NMR workshops & conferences, young investigators meetings, etc. The program website will be available soon and will be announced.
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The NMR 3D model of the new periplasmic Therm. Thermophilus protein (PCu(A)C) {Abriata LA. et al. Nature Chem Biol 2008, 599-601}. See more…
The simulated complex of CDK1 and newly synthesized pyrrolo-carbazoles {Fousteris MA. et al. J Med Chem 2008, 51, 1048-1052}. See more… 
Joint EUROMAR 2010 and 17th ISMAR Conference - A WorldWide Magnetic Resonance Conference is held at Florence, Italy, July 4-9, 2010.
Bentham Sci. “Current Pharmaceutical Design” has published a Special Issue with 9 articles on the Latest Achievements on Zn-enzymes (2009; Vol 15, Issue 31) . 
