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Georgios A. Spyroulias, PhD.Department of Pharmacy
University of Patras
Panepistimioupoli-Rion,
GR-26504 Patras, GREECE
Tel: +30.2610.969950 (office)
+30.2610.969951 (terra silico)
+30.2610.969952 (terra vitro)
Fax: +30.2610.969950
Email: G.A.Spyroulias@upatras.gr
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Profile
 Polypeptides tend to become organized independently into a variety of functional scaffolds and to socialize with other partner molecules, forming small biomolecular complexes or large assemblies, in various intersections of biological pathways. Proteins’ architecture, plasticity and interaction determine the fate of a variety of biological events. Understanding the functional role of peptides and proteins is strongly coupled to the atomic-level insights of their structure and flexibility.The research activities of the group encompass the study of the structure and dynamics of peptides and proteins involved in signalling pathways, proteolysis and ion/metal transfer, both in silico and in vitro. |
Terra Vitro
Terra Vitro is the wet biology lab for protein production, labelling, purification and characterization. NMR study of proteins and protein complexes requires the production and isolation of the molecules labelled in 13C and 15N active NMR nuclei. Proteins with a molecular weight ranging from 8 to 22 kDa (70 to 200 amino acids) are currently over-expressed in high yields in our lab using bacterial and eukaryotic systems and purified/isolated accordingly.
The lab offers a variety of expression vectors and is equipped with all the appropriate facilities for expression, purification and analysis of recombinant native or mutated proteins, bearing a variety of purification tags.
Terra Silico
Terra Silico is the computer lab for NMR data analysis and Biomolecular Simulations. NMR data provide an atomic-level information for the structure, dynamics, and interaction of polypeptides and can drive Structure-Activity Relationship studies through the design of protein/peptide analogues or protein substrates. Biomolecular Simulations are applied to study the conformational dynamics of protein-protein /peptide /substrate complexes.
Terra silico is equipped with powerful computational systems running software for NMR data analysis, structure calculations, docking and molecular dynamics simulation, structure validation etc.
GR-NMR
An NMR-related site presenting the Greek groups and their activities in the field. There is a number of teams in various universities and institutes that are applying NMR spectroscopy for conformational studies and for analytical purposes. NMR instruments are settled down in Universities of Athens, Thessaloniki, Ioannina, Crete and Patras, as well as research institutes at Athens (NCSR Demokritos, National Hellenic Research Foundation; NHRF) with the higher field magnet being 600 MHz (U of Athens and NHRF). The research activities of the Greek NMR groups are oriented to the analysis of natural products and drug candidates, the conformational properties of peptides and proteins and food quality analysis. The 1st National Greek NMR Meeting was held, as a Post-Symposium event to 18th Euro QSAR. For information about the roadmap of the BioNMR community in Greece, as resulted by this meeting, and to get the material of the meeting follow the link Read more...EAST-NMR
EAST-NMR is an FP7 EU-funded project. It has the specific aim to enhance the access to NMR facilities and the NMR services to East European users towards an efficient and coordinated pan-European pool of NMR capacities to enable collaborative research and technological achievements. EAST-NMR will combine activities, such as: (i) Networking, (ii) Trans-national access, and (iii) Joint research activities, in a co-ordinated manner. University of Patras is the Greek partner (Partner #12) to EAST-NMR project and is participating to a variety of activities such as, NMR workshops & conferences, young investigators meetings, etc. The program website will be available soon and will be announced.
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The NMR 3D model of the Arkadia E3 Ubiquitin ligase, a RING-H2 Finger domain determined recently and deposited to PDB {Chasapis C. et al. 2010}. 
In silico models of eleven dual inhibitors docked in the active sites of ACEC, ACEN and NEP proteases {Dimitropoulos N. et al. JCIM 2010, 50, 388-396}. 
In silico biomolecular complex of a1 nAChR bound to two toxins (a-bungarotoxin & a-cobratoxin) {Dimitropoulos N. et al. PROTEINS 2010, in the press} 
EAST-NMR Satellite meeting organized as a 18th EuroQSAR (19th - -24th September 2010) post-symposium event the 24th and 25th of September 2010, at Rhodes, Greece. Read more… 
EUROMAR 2011 the 33rd Discussion Meeting of the Magnetic Resonance Spectroscopy Division of the German Chemical Society, is held at Frankfurt am Main, Germany, August 21st - 25th, 2011. 
Bentham Sci. “Current Pharmaceutical Design” has published a Special Issue with 9 articles on the Latest Achievements on Zn-enzymes (2009; Vol 15, Issue 31). 
